General view of the Bone and Wheeler gas analysis apparatus used at the U.S. Department of Agriculture's Fixed Nitrogen Research Laboratory located in Washington, D.C. A Bone and Wheeler apparatus, which reads gas samples at a constant volume with pressure differences noted, notably lacks the large numbers of pipettes common in other gas analysis apparatus.

The Fixed Nitrogen Research Laboratory (F.N.R.L.) was established at American University in 1919 under the directorship of Arthur B. Lamb. Initially part of the War Department, the F.N.R.L. was the successor to several wartime initiatives to develop a secure domestic supply of nitrate compounds necessary for the manufacture of explosives during World War I. With a staff of about 110 individuals, including 35 to 50 chemists, the F.N.R.L. focused on the manufacture, production, and development of products of atmospheric nitrogen, including munitions and fertilizers.