Mildred Cohn begins the interview by reflecting on her childhood, education, and family life, describing how she was prepared to enter college by age fourteen. She then discusses her undergraduate experience at Hunter College, recalling the difficulties she encountered as a woman in the sciences. She continues by recounting her graduate years at Columbia, where, after working for a short time at the National Advisory Committee for Aeronautics, she began her work with isotopes in Urey's lab. She tells of her experience working with du Vigneaud at George Washington and Cornell universities and contrasts that with the much more independent atmosphere of the Cori's lab at Washington University in St. Louis. Finally she describes her years at the University of Pennsylvania and highlights the most fulfilling aspects of her work. She concludes with her analysis of the future of biochemistry and advice for those, especially women, interested in pursuing a career in the natural sciences.